Would you like to know how the original Salk polio vaccine was made?
1. Put all the glassware inside a hot steam bath or sterilize it.
2. Import Macaca mulatta monkeys from India for the experiments.
3. Prepare a mixture called “medium 199″ – containing 2 % calf serum, 200 units/ml penicillin, 200 ,g/ml dihydrostreptomycin and 50 units/ml Mycostatin (nystatin Squibb); the pH of the medium was brought to 7.0 by the addition of a NaHCO3 solution.” (full serum ingredients commented below)
4. Euthanize the monkeys, remove their kidneys, and then place the kidney into a tube and hand mince it with scissors into small bits.
5. After the kidney tissue was weighed and decapsulated, they put the tissue in a centrifuge tube where they washed it in a phosphate buffered saline and placed in a trypsinization flask. Trypsin enzymes break down the proteins, and is then centrifuged at 800-1000 RPM for 10 minutes to separate the tissue and cells.
6. The kidney cells are then mixed with the medium 199 and incubated (fermented, rotted essentially) at 37C for 6-8 days. By the end of the 6-8 day period, the bottles and tubes were covered with a “confluent sheet of cell growth”.
7. Once the medium 199 is exhausted, half is siphoned off to be replaced by fresh medium 199.
8. They then add the “polio virus” for the first time. 3 different strains supposedly, with no other details on source, isolation process, or genome determination (because that wasn’t discerned until 1981 when Dr. Vincent Racaniello and Dr. David Baltimore at MIT were said to have sequenced the virus)
9. The bottles continue to rock for 4 days in the solution culturing, fermenting, decaying, and then it’s ready for harvesting.
10. Scientists then visually look at the vials under a microscope to do a “titration test” to discern how much live virus is in the solution, hand counting particles that could be ANYTHING. (very scientific…)
11. Next is filtration, the most egregious part. They filter the solution first through porcelain filters (heavy metal risk), and then through MULTIPLE SHEETS OF ASBESTOS to drain out any kidney tissue or stray bacteria.
(This part of the process is not disclosed in the video, but is detailed in the original paper on the polio vaccine process. They made multiple trivalent vaccine pools, with some having additional additives like sodium bisulfite, along with parabens, a known carcinogen and endocrine disruptor)
12. Now rabbits, monkeys, guinea pigs, and chickens are injected with the “live virus” vaccine solutions (See page 11, 12, 13, in full paper) to ensure it’s free of other pathogens.
13. Now the “climax” of the process as they call it, inactivation. This is where they mix the vaccine solution with formaldehyde, and then let it sit together for 66-68 hours. The narrator then hilariously says “then what remains can only do good, can provide humans with protection of paralytic polio.” – “The enemy of man can now become his servant”.
Propaganda in 1900s just hits different doesn’t it?
14. Then the process of mass distribution. They get these massive tanks and mix in all the solutions, adjuvants, chemicals, and ingredients for mass production and “preservation”.
15. Before mass administration, they do a couple experiments on mice and monkeys to ensure the vaccine is creating enough “polio fighting antibodies” in humans.
The remainder of the process details the various “tests” they do as the vaccine lots are distributed, before really turning up the propaganda and showing President Eisenhower’s son receiving the polio vaccine.
Welcome to modern vaccinology. A hilariously unscientific process predicated on insane barbarism, rife with fraud and immense hubris.
This part from the paper…. “The discussion in the literature 17, 19 as to whether the inactivation of poliomyelitis virus by formaldehyde runs the course of a first-order chemical reaction or deviates from the linear course appears to us to be a theoretical rather than a practical problem. Since such a high degree of over-treatment with formalin is applied, the inactivation curves seemed to us non-relevant from the practical point of view. The general slope of the inactivation curve is undoubtedly important since it demonstrates whether the inactivation process is proceeding in the desired way. No dependence, however, can be placed on the precise rate of the initial fall in virus titre. The exact slope of the inactivation curves had no bearing on the ultimate outcome of the inactivation process, as was proved by subsequent safety tests carried out on the last day of inactivation and three days previously (see Fig. 7, page 1017). Consequently, the ultimate reliance for the safety of a vaccine must be the absence of live poliovirus as demonstrated by the safety tests of the monovalent and polyvalent pools.”
polio vaccine process1
Leave a Reply